Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Microbiota-driven therapeutic efficacy of Hyperoside in ulcerative colitis and associated anxiety

doi: 10.3389/fcimb.2026.1734356

Figure Lengend Snippet: Network pharmacology and molecular docking elucidate potential mechanisms of HYP in UC treatment. (A) Venn diagram of HYP and UC target genes. (B) PPI network of common targets. (C) KEGG pathway analysis. (D) The top 20 gene targets ranked by degree value. Molecular docking binding modes and affinities of HYP with (E) MAPK3, (F) AKT1, and (G) NFκB1. (H) Protein expression levels of p38MAPK, p-p38MAPK, AKT, p-AKT, NF-κB p65, and p-NF-κB p65 in the colon tissues.

Article Snippet: The following antibodies were used in this study: anti-ZO-1 antibody (AF5145, Affinity), anti-mucin-2 antibody (GB11344, Serbicebio), anti-BDNF antibody (GB11559, Serbicebio), anti-GFAP antibody (GB11096, Serbicebio), anti-Iba-1 antibody ( GB154490 , Serbicebio), NF-κB p65 (Proteintech, 10745-1-AP), Phospho-NF-κB p65 (Affinity, AF2006), AKT (Proteintech, 10176-2-AP), Phospho-AKT (Proteintech, 28731-1-AP), p38MAPK (Proteintech, 51115-1-AP), Phospho-p38MAPK (Affinity, AF4001).

Techniques: Binding Assay, Expressing

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Microbiota-driven therapeutic efficacy of Hyperoside in ulcerative colitis and associated anxiety

doi: 10.3389/fcimb.2026.1734356

Figure Lengend Snippet: Schematic of the mechanism by which HYP ameliorates DSS-induced UC and associated anxiety-like behaviors. HYP alleviates UC and comorbid anxiety through gut microbiota-dependent mechanisms. It remodels the gut microbial community, thereby enhancing host arginine metabolism and directly modulating the MAPK/PI3K-Akt/NF-κB signaling pathways. This integrated action suppresses systemic and neuroinflammation, restores intestinal barrier function, and ultimately ameliorates both colonic pathology and anxiety-like behaviors.

Article Snippet: The following antibodies were used in this study: anti-ZO-1 antibody (AF5145, Affinity), anti-mucin-2 antibody (GB11344, Serbicebio), anti-BDNF antibody (GB11559, Serbicebio), anti-GFAP antibody (GB11096, Serbicebio), anti-Iba-1 antibody ( GB154490 , Serbicebio), NF-κB p65 (Proteintech, 10745-1-AP), Phospho-NF-κB p65 (Affinity, AF2006), AKT (Proteintech, 10176-2-AP), Phospho-AKT (Proteintech, 28731-1-AP), p38MAPK (Proteintech, 51115-1-AP), Phospho-p38MAPK (Affinity, AF4001).

Techniques: Protein-Protein interactions

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: A Topical Chinese Herbal Alleviates Psoriasis by Regulating Keratinocytes Pyroptosis Through Inhibition of NLRP3 Inflammasome Activation

doi: 10.2147/CCID.S559712

Figure Lengend Snippet: ( A – C ) mRNA levels of NLRP3, Caspase-1, GSDMD in NHEK cells, n = 3. ( D – I ) Protein levels of NF-κB, P65, NLRP3, caspase-1, GSDMD in NHEK cells, n = 3.*P < 0.05, **P < 0.01, ***P < 0.001 compared with Control; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with Model; & P < 0.05, && P < 0.01, &&& P < 0.001 compared with QS; ^P < 0.05, ^^P < 0.01 compared with Mcc950.

Article Snippet: The sections were then incubated overnight at 4°C with the following primary antibodies: NF-κB p65 Monoclonal antibody (66535-1-Ig, Proteintech, China), NLRP3 Monoclonal antibody (68102-1-Ig-100, Proteintech, China), Caspase-1 antibody (sc-56036, Santa Cruz, USA), and GSDMD Polyclonal antibody (20770-1-AP, Proteintech, China).

Techniques: Control

Journal: Arthritis Research & Therapy

Article Title: Hyaluronic acid fragments enhance the inflammatory and catabolic response in human intervertebral disc cells through modulation of toll-like receptor 2 signalling pathways

doi: 10.1186/ar4274

Figure Lengend Snippet: The role of NF-κB in fHA-dependent IVD cell activation . ( A ) Immunofluorescence staining of intervertebral disc (IVD) cells for NF-κB (p65) (green) following treatment with either hyaluronic acid fragments (fHAs) (20 μg/ml) or interleukin (IL)-1β (5 ng/ml) at selected time points. Nuclear NF-κB (p65) is indicated by arrowheads. Untreated IVD cells served as a control. Scale bar = 50 μm. ( B ) Western blot analysis of nuclear extracts from untreated IVD cells or cells treated for 1 hour with either fHAs (20 μg/ml) or IL-1β (5 ng/ml). Levels of PARP1 protein served as a loading control. ( C ) NF-κB (p65) DNA binding activity in nuclear extracts from untreated IVD cells, or cells treated for one hour with IL-1β or fHAs (20 μg/ml) ( n = 3). In all cases, analyses were performed in triplicate and values expressed as mean ± S.D. Statistical analysis was performed using the Student's t -test, * P <0.01 as compared to untreated cells.

Article Snippet: Membranes were then incubated for 2 hours at room temperature with either anti- NF-κB (p65) (1:200) or anti-PARP1 (1:1,000) (both from LabForce, Switzerland).

Techniques: Activation Assay, Immunofluorescence, Staining, Control, Western Blot, Binding Assay, Activity Assay